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Image Search Results
Journal:
Article Title: Telomerase-Associated Protein TEP1 Is Not Essential for Telomerase Activity or Telomere Length Maintenance In Vivo
doi:
Figure Lengend Snippet: Disruption of the murine Tep1 gene in ES cells and mice. (A) Schematic representation of homologous recombination of the targeting vector with the endogenous locus. Insertion of a new Spe1 site into the targeted locus allows the targeted and wild-type alleles to be distinguished by Southern blot analysis with the indicated 3′ flanking probe. S, SpeI; N, Not1. (B) Detection of targeted and wild-type mTep1 alleles by Southern blot analysis of DNA from G1 mice. DNA was digested with SpeI and hybridized with the probe shown in panel A. The 5.1-kb SpeI fragment corresponding to the wild-type allele is decreased to 1.6 kb upon disruption of the locus by integration of the Neor gene. (C) Immunoblot of MEF extracts probed with an anti-TEP1 antibody (12). The position of the mTep1 protein is indicated. The first lane contains human 293 cell lysate transfected with a recombinant hTEP1 plasmid (293-hTEP1). The protein species below the 85-kDa protein marker in each immunoprecipitate is not specific to anti-TEP1 antibody.
Article Snippet: To isolate murine Tep1 genomic fragments, a
Techniques: Homologous Recombination, Plasmid Preparation, Southern Blot, Western Blot, Transfection, Recombinant, Marker